Auto Pap Smear

Pap stains incorporate a second stain component that interacts with the not fully reacted hematoxylin stain: The Phosphotungstic acid binds to fibrin & collagen, but will also bind to unreacted mordanted hematein.

The result is that the hematoxylin stain now has a larger impact than in just H&E alone. Since there is not an IHC follow up, the risk of a false negative diagnosis is a serious issue.

To ensure consistent diagnostic determination the hematoxylin stain reagent must be held to a consistent concentration, pH, and reactivity.

Using digital imaging, the image can be normalized by using the PRS-PAP target information to compensate for hematoxylin drift

The PRS-PAP utilizes chemical targets that uniquely fully react with a single stain component so that each stain component can be objectively evaluated.

In a QC modality, the PRS-PAP validates the stain reagents are working normally.